abstract：:Pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene, is associated with tumor formation, proliferation and invasiveness. F-PBF(beta-TrCP), a fusion protein, was produced by replacing the WD40-repeat of F-box protein beta-TrCP with the PTTG1-binding factor (PBF) for targeted degradation of PTTG1. To evaluate ...

journal_title：Journal of biotechnology

authors： Mo Z,Zu X,Xie Z,Li W,Ning H,Jiang Y,Xu W

更新日期：2009-01-01 00:00:00

abstract：:The grapevine genetic transformation programs have relayed on the use of solid media-based somatic embryogenesis. To reach a high throughput of candidate gene evaluation in 'Thompson Seedless', a semi-automatic system allowing viable transformation of explants was designed. An intermediate procedure using liquid media...

journal_title：Journal of biotechnology

authors： Tapia E,Sequeida A,Castro A,Montes C,Zamora P,López R,Acevedo F,Prieto H

更新日期：2009-01-01 00:00:00

abstract：:In the present study we show that recombinant bacterial CotA-laccase from Bacillus subtilis is able to decolourise, at alkaline pH and in the absence of redox mediators, a variety of structurally different synthetic dyes. The enzymatic biotransformation of the azo dye Sudan Orange G (SOG) was addressed in more detail ...

journal_title：Journal of biotechnology

authors： Pereira L,Coelho AV,Viegas CA,Santos MM,Robalo MP,Martins LO

更新日期：2009-01-01 00:00:00

abstract：:The kinetics of microbial growth and the biodegradation of methanol and toluene in (a) biofilters (BFs), and (b) biotrickling filters (BTFs), packed with inert materials, has been studied and analyzed. The specific growth rate, mu, for the treatment of methanol was 0.037h(-1) for a wide range of operating conditions. ...

journal_title：Journal of biotechnology

authors： Avalos Ramirez A,Bénard S,Giroir-Fendler A,Jones JP,Heitz M

更新日期：2008-11-25 00:00:00

abstract：:Escherichia coli strain BL21(DE3) was tested as a delivery vector for gene transfer to a murine P388D1 macrophage cell line using a 96-well high-throughput assay. Five recombinant strains of E. coli were compared to identify the effect recombinant listeriolysin O (LLO) and associated gene expression parameters had on ...

journal_title：Journal of biotechnology

authors： Parsa S,Wang Y,Rines K,Pfeifer BA

更新日期：2008-10-10 00:00:00

abstract：:The heterologous expression of streptococcal genes in common Gram-negative hosts may be complicated by low-level expression, toxicity to the host, formation of inclusion bodies, and mislocalization of the encoded proteins. Biochemical study of the Streptococcus agalactiae virulence-associated cell-envelope protease (C...

journal_title：Journal of biotechnology

authors： Shelver D,Bryan JD

更新日期：2008-09-10 00:00:00

abstract：:Two types of eukaryotic operon-type Expression clones were constructed using the Multisite Gateway system employing six types of att signals. These clones harbored a DNA cassette containing two heterologous ORFs (cDNAs) or three heterologous ORFs in tandem downstream of a single promoter. The most promoter-proximal OR...

journal_title：Journal of biotechnology

authors： Sasaki Y,Sone T,Yahata K,Kishine H,Hotta J,Chesnut JD,Honda T,Imamoto F

更新日期：2008-09-10 00:00:00

abstract：:European eel (Anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3',4,4'-tetrachlorobiyphenyl (PCB77) to examine and characterize the inductive effect of coplanar PCBs on CYP1A1 gene expression in liver and gills by using a semi-quantitative RT-PCR analysis. The influence of PCB77 inje...

journal_title：Journal of biotechnology

authors： Marohn L,Rehbein H,Kündiger R,Hanel R

更新日期：2008-09-10 00:00:00

abstract：:Composition and gene content of a biogas-producing microbial community from a production-scale biogas plant fed with renewable primary products was analysed by means of a metagenomic approach applying the ultrafast 454-pyrosequencing technology. Sequencing of isolated total community DNA on a Genome Sequencer FLX Syst...

journal_title：Journal of biotechnology

authors： Schlüter A,Bekel T,Diaz NN,Dondrup M,Eichenlaub R,Gartemann KH,Krahn I,Krause L,Krömeke H,Kruse O,Mussgnug JH,Neuweger H,Niehaus K,Pühler A,Runte KJ,Szczepanowski R,Tauch A,Tilker A,Viehöver P,Goesmann A

更新日期：2008-08-31 00:00:00

abstract：:The feed profile of glucose during fedbatch cultivation could be used to influence the retention of the periplasmic product ZZ-cutinase. An increased feed rate led to a higher production rate but also to an increased specific leakage, which reduced the periplasmic retention. Three growth rates: 0.3, 0.2 and 0.1 h(-1) ...

journal_title：Journal of biotechnology

authors： Bäcklund E,Reeks D,Markland K,Weir N,Bowering L,Larsson G

更新日期：2008-07-31 00:00:00

abstract：:The invariant surface glycoprotein ISG75 is a transmembrane glycoprotein occurring on the surface of the bloodstream-form Trypanozoon. This study describes the expression and purification of the N-terminal extracellular domain of ISG75, a novel target for development of diagnostic tests for trypanosomosis. To facilita...

journal_title：Journal of biotechnology

authors： Tran T,Büscher P,Vandenbussche G,Wyns L,Messens J,De Greve H

更新日期：2008-06-30 00:00:00

abstract：MOTIVATION:A set of genes and their gene expression levels are used to classify disease and normal tissues. Due to the massive number of genes in microarray, there are a large number of edges to divide different classes of genes in microarray space. The edging genes (EGs) can be co-regulated genes, they can also be on ...

journal_title：Journal of biotechnology

authors： An J,Chen YP

更新日期：2008-06-30 00:00:00

abstract：:The catalytic efficiency and pH optimum of Trichoderma reesei endo-beta-1,4-glucanase II were improved by protein engineering. We subjected residue 342 to saturation mutagenesis, and further changed the enzyme by random mutagenesis and two rounds of DNA shuffling. Enzyme variants were purified and characterized. Varia...

journal_title：Journal of biotechnology

authors： Qin Y,Wei X,Song X,Qu Y

更新日期：2008-06-01 00:00:00

abstract：:This study addresses the problem of poor expression of somatotropin (ST) gene in E. coli and describes expression enhancement through silent and non-silent gene modifications. A series of constructs with codon optimization, substitution, deletion or addition in the 5'-region of the sequence encoding bubaline ST (BbST)...

journal_title：Journal of biotechnology

authors： Sadaf S,Khan MA,Akhtar MW

更新日期：2008-06-01 00:00:00

abstract：:Detailed knowledge of mammalian cell culture proliferation kinetics is important to determine fed-batch strategies for industrial bioreactor operations. In particular, predicting the end of exponential proliferation in batch culture is a critical process parameter during culture scale-up. Using automated flow cytometr...

journal_title：Journal of biotechnology

authors： Sitton G,Srienc F

更新日期：2008-06-01 00:00:00

abstract：:A critical step in the development of robust Agrobacterium tumefaciens-mediated transformation system in recalcitrant grain legume, cowpea is the establishment of optimal conditions for efficient T-DNA delivery into target tissue and recovery of transgenic plants. A dramatic increase in efficiency of T-DNA delivery wa...

journal_title：Journal of biotechnology

authors： Solleti SK,Bakshi S,Sahoo L

更新日期：2008-05-20 00:00:00

abstract：:In earlier study from our group, cholera toxin B subunit had been expressed in tomato for developing a plant-based vaccine against cholera. In the present investigation, gene for accessory colonization factor (acf) subunit A, earlier reported to be essential for efficient colonization in the intestine, has been expres...

journal_title：Journal of biotechnology

authors： Sharma MK,Jani D,Thungapathra M,Gautam JK,Meena LS,Singh Y,Ghosh A,Tyagi AK,Sharma AK

更新日期：2008-05-20 00:00:00

abstract：:Mutants of transketolase (TK) with improved substrate specificity towards the non-natural aliphatic aldehyde substrate propionaldehyde have been obtained by directed evolution. We used the same active-site targeted saturation mutagenesis libraries from which we previously identified mutants with improved activity towa...

journal_title：Journal of biotechnology

authors： Hibbert EG,Senussi T,Smith ME,Costelloe SJ,Ward JM,Hailes HC,Dalby PA

更新日期：2008-04-30 00:00:00

abstract：:Hepatocyte growth factor (HGF) is biosynthesized as a biologically inactive, single-chain form (pro-HGF). Its activation is associated with cleavage at Arg494-Val495 into a two-chain mature form composed of disulfide-linked alpha- and beta-chains. Because serum is a major source of HGF activator (the predominant serin...

journal_title：Journal of biotechnology

authors： Hayata D,Fukuta K,Matsumoto K,Adachi E,Hanada K,Adachi K,Nakamura T

更新日期：2008-02-29 00:00:00

abstract：:Expanded bed adsorption chromatography is used to capture the protein product of interest from a crude biological suspension directly, thereby eliminating the need for the removal of the cell debris. While this technique may replace three or four unit operations in a typical downstream process for biological product r...

journal_title：Journal of biotechnology

authors： Balasundaram B,Harrison ST

更新日期：2008-02-01 00:00:00

abstract：:The efficiency of protein synthesis for glyceraldehyde-3-phosphate dehydrogenase (G3PDH) was examined with several in vitro coupled transcription/translation protein synthesis systems based on Escherichia coli lysate, wheat germ, or reticulocyte lysate, and an in vitro translation system based on wheat germ extract. A...

journal_title：Journal of biotechnology

authors： Hino M,Kataoka M,Kajimoto K,Yamamoto T,Kido J,Shinohara Y,Baba Y

更新日期：2008-01-20 00:00:00

abstract：:Biodegradable scaffolds are of great value in tissue engineering. We have developed a method for fabricating patient-specific vascular scaffolds from a biocompatible and biodegradable polymer, poly(L-lactide-co-epsilon-caprolactone). This method's usefulness is due to flexibility in the choice of materials and vascula...

journal_title：Journal of biotechnology

authors： Uchida T,Ikeda S,Oura H,Tada M,Nakano T,Fukuda T,Matsuda T,Negoro M,Arai F

更新日期：2008-01-20 00:00:00

abstract：:Porcine circovirus type 2 (PCV2) is considered to be associated with post-weaning multisystemic wasting syndrome (PMWS), which is a newly emerged economically important swine disease. The entire coding region of open reading frame 2 (ORF2), encoding the viral capsid protein (Cap), of PCV2 was cloned and sequenced from...

journal_title：Journal of biotechnology

authors： Wu PC,Chien MS,Tseng YY,Lin J,Lin WL,Yang CY,Huang C

更新日期：2008-01-01 00:00:00

abstract：:Functional screening for lipolytic enzymes from a metagenomic library (origin: Jae Sawn hot spring, Thailand) resulted in isolation of a novel patatin-like phospholipase (PLP) and an esterase (Est1). PLP contained four conserved domains similar to other patatin-like proteins with lipid acyl hydrolase activity. Likewis...

journal_title：Journal of biotechnology

authors： Tirawongsaroj P,Sriprang R,Harnpicharnchai P,Thongaram T,Champreda V,Tanapongpipat S,Pootanakit K,Eurwilaichitr L

更新日期：2008-01-01 00:00:00

abstract：:Directed evolution was used to expand the substrate specificity and functionality of glycine oxidase by using a high-throughput screening assay based on the 4-aminoantipyrine peroxidase system, with a coefficient of variance below 4%. After screening the library, one mutant with the desired changes was found. The muta...

journal_title：Journal of biotechnology

authors： Martínez-Martínez I,Navarro-Fernández J,García-Carmona F,Sánchez-Ferrer A

更新日期：2008-01-01 00:00:00

abstract：:Aspergillus niger AB1.13 cultures with glucoamylase production (with D-glucose as substrate) and without glucoamylase production (with D-xylose as substrate) were characterized by metabolic flux analysis. Two comprehensive metabolic models for d-glucose- as well as for D-xylose-consumption were used to quantify and co...

journal_title：Journal of biotechnology

authors： Melzer G,Dalpiaz A,Grote A,Kucklick M,Göcke Y,Jonas R,Dersch P,Franco-Lara E,Nörtemann B,Hempel DC

更新日期：2007-12-01 00:00:00

abstract：:The simultaneous biosynthesis of lovastatin (mevinolinic acid) and (+)-geodin by Aspergillus terreus ATCC 20542 with regard to the medium composition, i.e. the concentrations of carbon and nitrogen source, was described in this paper. A. terreus is a lovastatin producer but the formation of lovastatin was accompanied ...

journal_title：Journal of biotechnology

authors： Bizukojc M,Ledakowicz S

更新日期：2007-12-01 00:00:00

abstract：:New methods for an extended physiological characterization of yeast at a microtiter plate scale were applied to 27 deletion mutants of Saccharomyces cerevisiae cultivated on glucose and galactose as sole carbon sources. In this way, specific growth rates, specific rates of glucose consumption and ethanol production we...

journal_title：Journal of biotechnology

authors： Velagapudi VR,Wittmann C,Schneider K,Heinzle E

更新日期：2007-12-01 00:00:00

abstract：:The production of a mutant green fluorescent protein (S65TGFP), controlled by the maltose inducible glucoamylase promoter, was followed in situ in fed-batch cultures of recombinant Aspergillus niger using multi-wavelength fluorescence spectroscopy. Disturbance of quantitative product analysis by interfering fluorescen...

journal_title：Journal of biotechnology

authors： Ganzlin M,Marose S,Lu X,Hitzmann B,Scheper T,Rinas U

更新日期：2007-12-01 00:00:00

abstract：:Systems biology is an integrative science that aims at the global characterization of biological systems. Huge amounts of data regarding gene expression, proteins activity and metabolite concentrations are collected by designing systematic genetic or environmental perturbations. Then the challenge is to integrate such...

journal_title：Journal of biotechnology

authors： Teixeira AP,Carinhas N,Dias JM,Cruz P,Alves PM,Carrondo MJ,Oliveira R

更新日期：2007-12-01 00:00:00

abstract：:Fiber morphology and crystalline structure of poly[(R)-3-hydroxybutyrate] (P(3HB)) and stereocomplexed poly(lactide) (PLA) nanofibers were investigated by using scanning and transmission electron microscopies and X-ray and electron diffractions. In the P(3HB) nanofibers spun from less than 1 wt% 1,1,1,3,3,3-hexafluoro...

journal_title：Journal of biotechnology

authors： Ishii D,Lee WK,Kasuya K,Iwata T

更新日期：2007-11-01 00:00:00

abstract：:This study demonstrates the applicability of pressurized stirred tank bioreactors for oxygen transfer enhancement in aerobic cultivation processes. The specific power input and the reactor pressure was employed as process variable. As model organism Escherichia coli, Arxula adeninivorans, Saccharomyces cerevisiae and ...

journal_title：Journal of biotechnology

authors： Knoll A,Bartsch S,Husemann B,Engel P,Schroer K,Ribeiro B,Stöckmann C,Seletzky J,Büchs J

更新日期：2007-10-31 00:00:00

abstract：:The purification of human antibodies in an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) 6000 and phosphate was optimised by surface response methodology. A central composite design was used to evaluate the influence of phosphate, PEG and NaCl concentration and of the pH on the purity and extra...

journal_title：Journal of biotechnology

authors： Azevedo AM,Rosa PA,Ferreira IF,Aires-Barros MR

更新日期：2007-10-31 00:00:00

abstract：:Laccase from the white rot fungus Coriolopsis polyzona was immobilized for the first time through the formation of cross-linked enzyme aggregates (CLEAs). Laccase CLEAs were produced by using 1000g of polyethylene glycol per liter of enzyme solution as precipitant and 200muM of glutaraldehyde as a cross-linking agent....

journal_title：Journal of biotechnology

authors： Cabana H,Jones JP,Agathos SN

更新日期：2007-10-15 00:00:00

abstract：:The role of oligosaccharides as biotic elicitors for the enhanced production of antibiotics and enzymes has been established in recent years. These findings could have significant promise for the pharmaceutical and biotechnology industries. Before the elicitors could be used at large-scale, reproducible production and...

journal_title：Journal of biotechnology

authors： Murphy T,Roy I,Harrop A,Dixon K,Keshavarz T

更新日期：2007-09-30 00:00:00

abstract：:Hydrogen production by the dark fermentation of food wastes is an economic and environmentally friendly technology to produce the clean energy source as well as to treat the problematic wastes. However, the long-term operations of the continuous anaerobic reactor for fermentative hydrogen production were frequently un...

journal_title：Journal of biotechnology

authors： Jo JH,Jeon CO,Lee DS,Park JM

更新日期：2007-09-15 00:00:00

abstract：:In vitro display technologies, such as mRNA display and DNA display are powerful tools to screen peptides and proteins with desired functions from combinatorial libraries in the fields of directed protein evolution and proteomics. When screening combinatorial libraries of polypeptides (phenotype), each of which is dis...

journal_title：Journal of biotechnology

authors： Doi N,Takashima H,Wada A,Oishi Y,Nagano T,Yanagawa H

更新日期：2007-09-15 00:00:00

abstract：:Selected phage clones expressing a peptide with high binding affinity for recombinant human lactoferrin or von Willebrand factor (vWF) were covalently coupled to macroporous poly(dimethylacrylamide) monolithic column. Large pore size (10-100 microm) of macroporous poly(dimethylacrylamide) makes it possible to couple l...

journal_title：Journal of biotechnology

authors： Noppe W,Plieva FM,Vanhoorelbeke K,Deckmyn H,Tuncel M,Tuncel A,Galaev IY,Mattiasson B

更新日期：2007-09-15 00:00:00

abstract：:A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were ...

journal_title：Journal of biotechnology

authors： Dinu D,Nechifor MT,Stoian G,Costache M,Dinischiotu A

更新日期：2007-08-31 00:00:00

abstract：:This work is related to the set-up of overflowing exponential fed-batch cultures (O-EFBC) derived from carbon limited EFBC dedicated to the production of mycosubtilin, an antifungal lipopeptide belonging to the iturin family. O-EFBC permits the continuous removal of the product from the bioreactor achieving a complete...

journal_title：Journal of biotechnology

authors： Guez JS,Chenikher S,Cassar JP,Jacques P

更新日期：2007-08-01 00:00:00